Interactive effects of antioxidant vitamins and NOS3 genetic polymorphisms for breast cancer risk in Korean women Sang-Ah Lee, Kyoung-Mu Lee, Keun-Young Yoo, Dong-Young Noh, Sei-Hyun Ahn, Yong Shin, Daehee Kang. National Cancer Center Research Institute, Goyong-Si, Republic of Korea, Seoul National University College of Medicine, Seoul, Republic of Korea, Ulsan University College of Medicine, Seoul, Republic of Korea. To evaluate the interactive effect of antioxidant vitamins intake and NOS3 genetic polymorphisms on the risk of breast cancer, a case-control study was conducted in Korea. Histologically confirmed breast cancer cases (n=950) and age and menopause status-matched controls (n=950) with no present or previous history of cancer were recruited from several teaching hospitals in Seoul during 1998-2003. Dietary information was collected by trained interviewer with semi-quantitative food frequency questionnaire which was validated against three day 24-hour recall method. 454 cases and 566 controls provided dietary information. Two genetic polymorphisms of NOS3 (Glu298Asp (G>T) and T-786C) were determined by single base extension assay. To evaluate the interactive effects with dietary factors, diplotypes were constructed by combinations of haplotypes ([TG:TG] vs. [TG:others] + [others:others]). Odds ratios and 95% confidence intervals were estimated by unconditional logistic regression model adjusting for age, family history of breast cancer, total estrogen exposure, age of first full term pregnancy, hospital, and energy intake. There was no significant difference in energy intake between cases and controls. However, the consumption of vitamin A (OR=0.7, 95% CI=0.49-0.87), vitamin C (OR=0.7, 95% CI=0.51-0.87), vitamin E (OR=0.6, 95% CI=0.42-0.82), and folate (OR=0.3, 95% CI=0.21-0.48) significantly decreased the risk of breast cancer. NOS3 T-786C was significantly different between cases and controls (OR=0.5, 95% CI=0.30-0.85). However, NOS3 Glu298Asp (G>T) was not different. Total 7 haplotype of NOS3 were estimated from the genotype data; the most common diplotype was TG-TG (65.5%). Those women who intake less antioxidant vitamins with [TG:TG] diplotype of NOS3 had increased the risk of breast cancer compared with women with more antioxidant vitamins intake with other diplotypes (OR=2.1, 95% CI=1.14-3.71): for vitamin A, OR=1.8 (95% CI=1.02-3.16); vitamin C, OR=2.2 (95% CI=1.28-3.72); vitamin E, OR=2.2 (95% CI=1.19-4.04); folate, OR=1.9 (95% CI=1.08-3.37), respectively. However, the multiplicative interactions were not significant. Our results thus suggest that genetic polymorphisms of NOS3 and antioxidant vitamins intake might have the interactive effect on breast cancer risk. Abstract 5814, AACR 2005 Remember we are NOT Doctors and have NO medical training. This site is like an Encylopedia - there are many pages, many links on many topics. Support our work with any size DONATION - see left side of any page - for how to donate. You can help raise awareness of CAM.